About Nanopore-based typing

Oxford Nanopore has developed a new generation of sensing technology that uses nanopores embedded in high tech electronics to perform precise molecular analysis. When a DNA fragment is entering a nanopore, each DNA base is disrupting the electrical field with a specific signature and can be used as a single molecule detector. The deconvolution of the electrical signal is done using a basecaller converting the electric signal into a DNA sequence with a FASTQ output format, similar to Illumina based sequencing. This FASTQ file is then imported into HLA Twin 5.0 for genotyping. One major advantage of Nanopore sequencing is the long reads facilitating the phasing of even long-distant SNPs resulting in fewer ambiguities.

“This technology may represent a paradigm shift similar to Luminex Technology for Antibody assessment in the early / mid 2000’s”

Competitive Advantages

  • The fastest sequencing-based HLA genotyping method
  • Minimal capital cost
  • Simplified workflow compared to classical NGS
  • Higher resolution compared to SSO/SBT/ Real Time PCR
  • Turn around Time from DNA to results:
    -For deceased donors: < 6 hours
    -For routine typing for 12 samples: < 24 hours
  • Flexible and scalable based on laboratory requirements

This product is Research Use Only.