About Nanopore-based typing

Oxford Nanopore has developed a new generation of sensing technology that uses nanopores embedded in high tech electronics to perform precise molecular analysis. When a DNA fragment is entering a nanopore, each DNA base is disrupting the electrical field with a specific signature and can be used as a single molecule detector. The deconvolution of the electrical signal is done using a basecaller converting the electric signal into a DNA sequence with a FASTQ output format, similar to Illumina based sequencing. This FASTQ file is then imported into NANOTYPER™ for genotyping. One major advantage of Nanopore sequencing is the long reads facilitating the phasing of even long-distant SNPs resulting in fewer ambiguities.

“This technology may represent a paradigm shift similar to Luminex Technology for Antibody assessment in the early / mid 2000’s”

Competitive Advantages

  • The fastest sequencing-based HLA genotyping method
  • Minimal capital cost
  • Simplified workflow compared to classical NGS
  • Higher resolution compared to SSO/SBT/ Real Time PCR
  • Turn around Time from DNA to results:
    -For deceased donors: < 6 hours
    -For routine typing for 12 samples: < 24 hours
  • Flexible and scalable based on laboratory requirements

This product is Research Use Only.