AzureSeq 200 CE

The AzureSeq is a CE, resp. RUO RT-qPCR test intended for the qualitative detection of nucleic acid from the 2019-nCoV in nasopharyngeal (NP) and oropharyngeal (OP) swabs from individuals with signs and symptoms of infection who are suspected of COVID-19. The assay can be used in combination either with an RNA extracted sample (indirect method) or directly with a sample collected into the viral transport medium (VTM).

Product Highlights

Regulatory Status CE-IVD
Extraction Principle Direct Extraction
Target Genes N1, N2 capsid and RNAseP
Turn Around Time From Sample to Result Less than 120 min
Sensitivity Low copy number (0.5 copies/μL)
Number of Pipetting Steps 8 steps
Instrument Compatibility BioRad CFX96, Roche Lightcycler 480 System and QuantStudio 3, 5, and 7 Pro

Value Added

  • Enable daily processing of hundreds of samples
  • Accurate detection with reduced complexity, time and waste
  • User-friendly, custom formats
  • Increase your throughput 25% to 300%
  • Fewer steps reduce potential user error
  • Excellent for automation

Detection Principle

The RT-qPCR Mastermix contains primers to amplify the specific genes regions of the virus and probes designed to anneal to a target sequence located between the forward and reverse primers. The probes contain a reporter dye at the 5’ end and a quencher dye at the 3’ end. When the reporter and quencher dye are in close vicinity, the fluorescence of the reporter dye is quenched by the quencher with very low fluorescence emission. During the extension phase of the PCR cycle, the polymerase will hydrolase the reporter dye, which will be released to emit a strong fluorescence. This fluorescence will increase by each cycle of polymerisation.


Direct Extraction Method

Kit Compatibility Format

Product Code (REF)
20 uL reactions
AzureSeq - 200 CE 200 In Solution: InhibiTAQ Plus HotStart Mastermix, RTScript, Target Specific Primers and Probes