The Economics of NGS: A Cost Comparison of NGS Implementations with Legacy Technologies

06/06/2016

Authors: Nóra Nagy, Efi Melista, Krisztina Rigó, Peter Meintjes, Tim Hague Abstract: Recent technological advances in sequencing and related library preparation methods, cumulatively called Next Generation Sequencing (NGS) have provided new opportunities that allow the complete characterisation of the HLA genes in haploid fashion and are beginning to make their...

Holotype HLA Early Access Program Results: An “Uncontrolled” Study

26/05/2016

Authors: Efi Melista, Krisztina Rigó, Szilveszter Juhos, György Horváth, Peter Meintjes, Tim Hague Abstract: NGS-based (Next Generation Sequencing) HLA typing can offer accurate and unambiguous genotyping results with minimal manual intervention, eliminating the requirement for reflexive testing. In a clinical setting an easy-to-follow protocol with minimal hands-on time is also...

Towards a New Gold Standard – NGS Corrections to Sanger SBT Genotyping Results

13/10/2015

Authors: Efi Melista, Krisztina Rigo, Agnes Pasztor, Mette Christiansen, Francesca Eleonora Bertinetto, Peter Meintjes, Tim Hague Aim: HLA typing is a crucial step prior to transplantation, particularly for bone marrow transplants. Correct allele matching is essential in order to increase the success of transplantation outcomes and decrease the rate...

Diversity of MHC Genes in the 1000 Genomes Dataset

27/07/2015

Authors: Tünde Vágó, Péter Tóth, Tim Hague, Szilveszter Juhos Introduction: The 1000 Genomes Project (1KG) was the first large-scale sequencing project to obtain comparable whole genome sequences from diverse human populations. Previously we validated our HLA typing method using these datasets for Class-II and Class-II HLA genes for 3 fields accuracy. Furthermore, using...