Although BLAST (Basic Local Alignment Search Tool) is not strictly a next generation sequencing tool, I use it regularly for a wide variety of tasks. Here are a few scenarios I use BLAST for:
Year: 2013
Flashcard Fridays – How accurate is next generation sequencing?
As for most unspecific questions, my best answer is “it depends”.* And it depends on a lot of things: the sequencing platform, the experiment design, the lab protocol, the quality of the biological sample, the mood of the lab tech, etc. I could go on and on and on and on and on.
Workflow Wednesdays – Part 3. Read preprocessing – Filtering
After last weeks post about randomly subsampling fastq files, let’s take a look at a different method for making read files smaller.
Bioinformatics for Beginners – How to find known variants?
If you’re interested in a specific gene (or set of genes) of the human genome, one of the important question you might consider is what (and what kind of and how many) variants are usually found in that particular genomic region.
So here’s a list of online resources, to help you with this task. Feel free to add additional resources in the comments!
Flashcard Fridays – Open Online Courses
I was searching for online learning resources and ran into the following article:
“An Online Bioinformatics Curriculum” , written by David B. Searls. It is basically a collection of free online lectures and courses about biology, mathematics and computer science.