Although BLAST (Basic Local Alignment Search Tool) is not strictly a next generation sequencing tool, I use it regularly for a wide variety of tasks. Here are a few scenarios I use BLAST for:
Month: August 2013
Flashcard Fridays – How accurate is next generation sequencing?
As for most unspecific questions, my best answer is “it depends”.* And it depends on a lot of things: the sequencing platform, the experiment design, the lab protocol, the quality of the biological sample, the mood of the lab tech, etc. I could go on and on and on and on and on.