Holotype HLA™

Holotype HLA downloads are available for your convenience

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- Instructions for Use

- Known Product Limitations

- Workbooks

- Release Notes

 

No.
Product Reference
Protocol Version
Relevant IFU Version
1 H32.1 Protocol 2.1 Holotype HLA 96/7 CE v2 - Protocol 2.1 - IFUv12
2 H34.1 Protocol 2.1 Holotype HLA 96/7 CE v2 - Protocol 2.1 - IFUv12
3 H52.1 Protocol 2.1 Holotype HLA 24/7 CE v2 - Protocol 2.1 - IFUv11
4 H56 Protocol 2.1 Holotype HLA 24/7 CE v2 - Protocol 2.1 - IFUv11
5 H58 Protocol 2.1 Holotype HLA 24/7 CE v2 - Protocol 2.1 - IFUv11
6 H60 Protocol 2.1 Holotype HLA 24/7 CE v2 - Protocol 2.1 - IFUv11
7 H62 Protocol 2.1 Holotype HLA 24/11 CE v2 - Protocol 2.1 - IFUv3
8 H64 Protocol 2.1 Holotype HLA 24/11 CE v2 - Protocol 2.1 - IFUv3
9 H66 Protocol 2.1 Holotype HLA 24/11 CE v2 - Protocol 2.1 - IFUv3
10 H68 Protocol 2.1 Holotype HLA 24/11 CE v2 - Protocol 2.1 - IFUv3
11 H72 Protocol 2.1 Holotype HLA 96/11 CE v2 - Protocol 2.1 - IFUv3
12 H76 Protocol 2.1 Holotype HLA 96/11 CE v2 - Protocol 2.1 - IFUv3
13 H52.1 Protocol 3.0.1 Holotype HLA 24/7 CE v2 - Protocol 3.0.1 – IFUv1
14 H56 Protocol 3.0.1 Holotype HLA 24/7 CE v2 - Protocol 3.0.1 – IFUv1
15 H58 Protocol 3.0.1 Holotype HLA 24/7 CE v2 - Protocol 3.0.1 – IFUv1
16 H60 Protocol 3.0.1 Holotype HLA 24/7 CE v2 - Protocol 3.0.1 – IFUv1
17 H62 Protocol 3.0.1 Holotype HLA 24/11 CE v2 - Protocol 3.0.1 – IFUv1
18 H64 Protocol 3.0.1 Holotype HLA 24/11 CE v2 - Protocol 3.0.1 – IFUv1
19 H66 Protocol 3.0.1 Holotype HLA 24/11 CE v2 - Protocol 3.0.1 – IFUv1
20 H68 Protocol 3.0.1 Holotype HLA 24/11 CE v2 - Protocol 3.0.1 – IFUv1
21 H23 Protocol 3.0.1 Holotype HLA 96/5 CE v2 - Protocol 3.0.1 – IFUv1
22 H24 Protocol 3.0.1 Holotype HLA 96/5 CE v2 - Protocol 3.0.1 – IFUv1
23 H26 Protocol 3.0.1 Holotype HLA 96/5 CE v2 - Protocol 3.0.1 – IFUv1
24 H32.1 Protocol 3.0.1 Holotype HLA 96/7 CE v2 - Protocol 3.0.1 – IFUv1
25 H34.1 Protocol 3.0.1 Holotype HLA 96/7 CE v2 - Protocol 3.0.1 – IFUv1
26 H38 Protocol 3.0.1 Holotype HLA 96/7 CE v2 - Protocol 3.0.1 – IFUv1
27 H72 Protocol 3.0.1 Holotype HLA 96/11 CE v2 - Protocol 3.0.1 – IFUv1
28 H76 Protocol 3.0.1 Holotype HLA 96/11 CE v2 - Protocol 3.0.1 – IFUv1
29 H78 Protocol 3.0.1 Holotype HLA 96/11 CE v2 - Protocol 3.0.1 – IFUv1
Instructions for Use in Foreign Languages

Bulgarian

Protocol version 2.1

Czech

Protocol version 2.1

Danish

Protocol version 2.1

French

Protocol version 3.0.1

Protocol version 2.1

German

Protocol version 2.1

Greek

Protocol version 3.0.1

Protocol version 2.1

Italian

Protocol version 3.0.1

Protocol version 2.1

Portuguese 

Protocol version 2.1

Romanian

Protocol version 3.0.1

Protocol version 2.1

Spanish

Protocol version 2.1

Known Product Limitations

Release Notes

Release Notes for Holotype HLA v2.0 IFU v3.0.1

  • Decreased input DNA
  • Long range polymerase changed to Promega GoTaq
  • Enhancer 2 removed from protocol
  • New Amplification Master Mix volumes
  • New PCR Cycling conditions
  • ExoSAP purification of HLA amplicons was removed
  • AMpure magnetic bead purification of HLA amplicons was added
  • Modified Amplicon Pooling volumes
  • New Fragmentation Master Mix volumes
  • Size selection using Ampure XP magnetic beads supported
  • Library quantitation by Qubit was refined
  • Specified storage times