There is no strict request on the choice of the extraction method. The only requirement is the quality of the genomic DNA which must meet with the general recommendation for DNA quality for long-range PCR (A260/280 in range 1.8 – 2.0 and A260/230 in range 2.0 – 2.2). It is users responsibility to validate the assay with their gDNA samples.

We may not recommend using Prepito DNA Blood 400 Kit (CMG-2003) or Chemagic DNA Blood Kit (CMG-1086), NucleoMag Blood 200 µL or MagDEA DX extraction kit as we observed a negative interference with the PCR.

On the other hand, we may recommend the following extraction methods that have been internally successfully tested:

• Chemagic DNA Blood KIT (CMG-1086-EFS)from Revvity
• QIAamp Blood Mini Kit (250) (51106) from Qiagen
• Maxwell RSC Whole Blood DNA Kit (AS1520) from Promega
• NucleoSpin Blood (740951.50) from Machery-Nagel