You can always try to run the flow cell again without reloading the library because there still may be some unsequenced library on the chip. Simply keep the flow cell with the library in MinION and set up another run. This will generate a new output folder, however, barcodes will be identical as in the previous run. So you can then simply combine the fastq files of each sample after the run with the ones from the previous run and repeat the analysis.

Refueling of the system with fresh buffer might be requires. As a general rule, we recommend that if the translocation speed drops below 300b/s then this is a good time to consider refuelling. Follow the instruction on ONT Community channel in the article titled “Refuelling your flow cell”.