There are three safe stopping points in the protocol for storing the library:

1. After the barcoding reaction,
2. After the library pooling and purification,
3. And after the adapter attachment.

In case of points 1 and 2, the recommended storage conditions are 4°C for up to 1 month and for long-term storage, we recommend storage temperatures less than -80°C.

Although libraries should be immediately sequenced after the attachment of the adapter (point 3), they can also be stored at 4°C for up to 1 week if needed.,

After adding sequencing buffer and loading beads to the library, there’s no option for storing the library. Such library must be immediately loaded to the flow cell.

If something happens which prevents to start the sequencing and the library has been already loaded to the flow cell it is safe to store the flow cell in the fridge (4-8°C) for max 24 hours.