The FLUSH BUFFER (FB) vial contains 15ml of FB. The FB contains ATP which is sensitive to freeze/thaw cycles. We recommend to make aliquotes to use only 1 vial each time.
1.Take 12 pieces of 1.5ml tubes,
2.Aliquot 1.17ml from the Flush Buffer (FB) in each of the tubes,
3.Freeze the tubes,
4.Use 1 tube of 1.17ml FB each time you do flow cell priming.